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  • Chem Sci Trans., 2015, 4(2),  pp 377-388  

    DOI:10.7598/cst2015.1020

    Research Article

    Simple and Improved Visible Spectrophotometric Method for Determination of Paracetamol Using Sodium Nitroprusside Chromogenic Reagent

  • SHIVAJI R. LABHADE1*, KAILAS R. LABHADE2 and VISHWAS B. GAIKWAD3
  • 1Department of Chemistry, K.T.H.M. College, Gangapur Road, Shivajinagar, Nashik- 422 002, India
    2Department of Chemistry, S.V.K.T. Arts, Science and Commerce College, Deolali Camp, Nashik- 422 401, India
    3Director, BCUD, Savitribai Phule Pune University, Ganeshkhind, Pune-411 007, Maharashtra State, India
  • Abstract

    A simple, rapid and improved visible spectrophotometric method has been proposed for determination of paracetamol (N-acetyl-p-aminophenol) in bulk drug. The experiment is based on the alkaline hydrolysis of paracetamol (PCT) to p-aminophenol (PAP) at elevated temperature and subsequent derivatization of PAP with sodium nitroprusside (SNP) at room temperature to produce an intense green colored N-nitrosyl-O-nitrosyl-p-aminophenol chromophore for direct determination of PCT at 685 nm. The molar absorptivity and Sandellís sensitivity are observed as 5.933x104 L.mol-1 .cm-1 and 0.0252 μg. cm-2 respectively for the chromophore. Beerís law is obeyed over the concentration range of 15-120 μg with linear regression equation as A= 0.009+0.003C (μg at 10 mL dilution). The percent recovery was found in the range 100.07-100.60%. The average of relative error and relative standard deviation are observed as 0.252% and 0.406% respectively. Reaction mechanism and reaction stoichiometry of the chromophoric reaction are discussed. The parameters with regard to determination of paracetamol by proposed method are optimized. Drugs like ibuprofen, aspirin and metformin do not interfere hence the proposed method is suitable for determination of PCT impurity in the said drugs.

    Keywords

    Paracetamol, N-acetyl-p-aminophenol, p-Aminophenol, N-nitrosyl-O-nitrosyl-p-aminophenol, Sodium nitroprusside

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