Diacetylmonoxime-3-amino-4-hydroxy benzoyl hydrazone (DMAHBH) was used as a novel chromogenic organic reagent for the determination of copper(II) by spectrophotometry. The novel chromogenic organic reagent diacetylmonoxime-3-amino-4-hydroxy benzoyl hydrazone (DMAHBH) gave bright yellow coloured water soluble complex with Cu(II) in basic buffer (pH 8.0-10.0) medium. The colour complex shows maximum absorbance at 412 nm. The system obeyed Beer's law in the concentration range of 0.3178 to 3.813 μ g/mL. The optimum copper(II) concentration range for accurate determination as evaluated from Ringbom?s plot was 0.6355 to 3.4953 μ g/mL. The molar absorptivity and Sandell's sensitivity were 1.65x104 L.mol-1cm-1 and 0.00606 μ g/cm2 respectively. The copper(II) forms M:L (I:I) colour complex with DMAHBH and stability constant of the complex was found to be. 7.15x105. This developed method was applied for the determination of copper(II) in food samples like vegetables and milk samples and obtained with good results compared with reference AAS results.
Novel chromogenic organic reagent, Derivative spectrophotometry, Cu(II) Food samples, Milk samples