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  • Chem Sci Trans., 2015, 4(3),  pp 844-850  

    DOI:10.7598/cst2015.1078

    Research Article

    Development of Analytical Method Protocol for the Separation and Analysis of Aflatoxins in Dry Coconut by RP-HPLC-UV

  • SHRIKAANT KULKARNI1, SHRIDHAR SAPTALE2, SHUJAT QUADRI4, MADHUKAR PATIL3, FAOZIA QUADRI4 and MAQDOOM FAROOQUI4
  • 1Department of Chemical Engineering, Vishwakarma Institute of Technology, Pune, India
    2Sinhgad Academy of Engineering, Kondhwa (Bk.), Pune, India
    3Yashwantrao Chavan College of Arts, Commerce & Science, Sillod, Aurangabad, India
    4Maulana Azad College of Arts, Commerce & Science, Aurangabad, India
  • Abstract

    Aflatoxins are mycotoxins, structurally related compounds produced as secondary metabolites by aspergillus molds, primarily flavus and parasiticus. Aflatoxins occur naturally in dry coconut, peanuts, cottonseed, corn, almond, cashew nut and dried chili pepper, as well as many mixed or processed foods and feeds. A simple, sensitive, and robust HPLC method and ultraviolet detection was used to analyze aflatoxins B1, B2, G1 and G2 in dry coconut. Although more number of aflatoxins exists, the four major toxins of interest are B1, B2, G1 and G2. They are designated according to their absorption properties in the UV region of the spectrum. Aflatoxin B1 and B2 emit radiation corresponding to blue wavelength, while G1 and G2 emit yellow-green wavelength. The extracts were cleaned using solid phase extraction method (SPE) preferably over immuno affinity columns (IAC) as the former give better recovery of aflatoxins than the latter one even with most challenging matrices and other methods like soxhlet extraction and accelerated solvent extraction. Further no interferences were found to be present after the cleanup of the sample matrix.

    Keywords

    Mycotoxins, Aflatoxins, Molds, RP-HPLC, Vomitoxin, A. Flavus, Fusarium

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