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  • Chem Sci Trans., 2015, 4(4),  pp 1121-1130  

    DOI:10.7598/cst2015.1137

    Research Article

    Development and Validation of HPTLC Method for Quantification of Biomarker β-Sitosterol in the Leaves of Achyranthes aspera Linn.

  • APARNA SARAF* and ARUNA SAMANT
  • The Institute of Science, 15, Madame Cama Road, Fort, Mumbai-400 032, India
  • Abstract

    A simple and sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the evaluation of biomarker β- sitosterol in the leaves of Achyranthes aspera Linn. belonging to family Amaranthaceae. Chromatography was performed on silica gel 60 F254 precoated HPTLC plates with solvents Toluene: Ethyl acetate: Glacial acetic acid 14.5: 4.5: 1.0 (v/v/v) as the mobile phase. After development, the HPTLC plate was derivatized with anisaldehyde sulphuric acid, scanned, and quantified at 540 nm. The system was found to give compact spot for β- sitosterol at RF = 0.61?0.02. The mean of %RSD value (n = 6) in Intra-day and Inter-day precisions studies for β- sitosterol were found to be 0.04% and 0.09%, respectively. The concentration of β- sitosterol in leaf of the plant is found to be 0.699 ng/μg. The statistical analysis proved that the developed method is suitable and specific. The developed method can be used as an important tool to assure the therapeutic dose of active ingredients in herbal formulations as well as for standardization and quality control of bulk drugs and in-process formulations.

    Keywords

    Achyranthes aspera Linn., β- sitosterol, HPTLC, Quantification, Validation

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