One of the most impressive finding in the field of chemoprevention is to prevent the occurrence of cancer by modulating the biotransformation of carcinogens. Our study was designed to elucidate the possible mechanism of chemoprevention by Gynandropsis gynandra through the biotransformation of unmetabolized aflatoxin B1 (AFB1) excretion in urine, distribution in liver by the phase I and phase II detoxification enzymes and the prevention of DNA damage caused by AFB1. The animals were pre-treated with the extract of Gynandropsis gynandra for seven days followed by a single injection of AFB1 dissolved in dimethyl sulphoxide (DMSO). The urine samples were collected on days one and three after AFB1 injection and analyzed for the unmetabolized AFB1 concentration. On day three after the injection, unmetabolized AFB1 and GSH content in the liver was measured followed by the activities of Cyt-P450, GST and QR. Pretreatment with the drug showed an enhanced rate of unmetabolized AFB1 excretion in the urine and a diminished distribution in the liver with maintained activities of the phase I and II enzymes. These results indicate that G.gynandra extract affords a potent chemoprevention against AFB1 through modulating the rate of biotransformation and detoxification and further prevented the DNA damage that was observed in AFB1 induced male albino rats.
Chemoprevention, Biotransformation, Aflatoxin B1, G. gynandra.