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  • Chem Sci Trans., 2016, 5(4),  pp 906-911  

    DOI:10.7598/cst2016.1275

    Research Article

    Biosynthesis of Silver Nanoparticles Using Saccharomyces Cerevisiae with Different pH and Study of Antimicrobial Activity against Bacterial Pathogens

  • M. SHEIK MUHIDEEN BADHUSHA* and M.M. ABDUL KADER MOHIDEEN,
  • Department of Chemistry, Sadakathullah Appa College, Tirunelveli, Tamilnadu, India
    Department of Microbiology, Sadakathullah Appa College, Tirunelveli, Tamilnadu, India
  • Abstract

    Extracellular biosynthesis of silver nanoparticles (Ag-NPs) using the Saccharomyces cerevisiae (Yeast) was carried out. The pH of the medium play a vital role in the synthesis of control shaped and sized nanoparticles. Morphological observation and characterization of biosynthesized silver nanoparticles were performed by UV-Visible spectroscopy, Scanning electron microscopy and Fourier transform infrared spectroscopy. The biosynthesized silver nanoparticles showed a maximum absorption in the visible region Saccharomyces cerevisiae strains showed a maximum absorption at 420-460 nm respectively and the size was ranged from 60-110 nm and 10-40 nm respectively. The antibacterial activities of silver nanoparticles (Ag-NPs) were studied with Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). The silver nanoparticles were synthesized at pH 6 that showed maximum antibacterial activity. This method is a promising eco-friendly alternative to chemical method

    Keywords

    Saccharomyces cerevisiae, Biosynthesis, Extracellular synthesis, Nanoparticles, Antimicrobial activity

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