Abstract

Extracellular biosynthesis of silver nanoparticles (Ag-NPs) using the Saccharomyces cerevisiae (Yeast) was carried out. The pH of the medium play a vital role in the synthesis of control shaped and sized nanoparticles. Morphological observation and characterization of biosynthesized silver nanoparticles were performed by UV-Visible spectroscopy, Scanning electron microscopy and Fourier transform infrared spectroscopy. The biosynthesized silver nanoparticles showed a maximum absorption in the visible region Saccharomyces cerevisiae strains showed a maximum absorption at 420-460 nm respectively and the size was ranged from 60-110 nm and 10-40 nm respectively. The antibacterial activities of silver nanoparticles (Ag-NPs) were studied with Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). The silver nanoparticles were synthesized at pH 6 that showed maximum antibacterial activity. This method is a promising eco-friendly alternative to chemical method

Keywords

Saccharomyces cerevisiae, Biosynthesis, Extracellular synthesis, Nanoparticles, Antimicrobial activity