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  • Chem Sci Trans., 2017, 6(1),  pp 42-49  

    DOI:10.7598/cst2017.1307

    Research Article

    Development and Validation of HPTLC Method for Quantification of Biomarker β-Sitosterol in Derris heyneana (Wight and Arn) Benth

  • POOJA SHINDE and APARNA SARAF*
  • Department of Botany, The Institute of Science, 15 Madame Cama Road, Fort, Mumbai 32, India
  • Abstract

    Derris heyneana (Wight and Arn) Benth is a medicinal woody climber belonging to family fabaceae found in Western Ghats of Maharashtra. There are no reports of quantification of β-sitosterol from this plant. Hence, a simple and sensitive High-Performance Thin-Layer Chromatographic (HPTLC) method was developed for identification and quantification of biomarker β-sitosterol in different parts of Derris heyneana. HPTLC was carried out with mobile phase Toluene: Ethyl acetate: Glacial Acetic acid: formic acid (12:6.1:1 (v/v)) on precoated aluminium silica gel plates (Merck) and densitometric determinations were done at 540 after derivatization with anisaldehyde sulphuric acid. Validation of β-sitosterol was done for methanolic stem extracts. Highest amount of β-sitosterol was found in stem. The method was validated using ICH guidelines in terms of Linearity, precision, specificity and accuracy. The linearity range was found to be 0.1 μg to 0.7 μ g/ spot

    Keywords

    β-sitosterol, Derris heyneana, Wight and Arn, Validation

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