• image 01
    Volume 1, No.1, 2012
  • image 02
    Volume 1, No.2, 2012
  • image 01
    Volume 1, No.3, 2012
  • image 02
    Volume 2, No.1, 2013
  • image 02
    Volume 2, No.2, 2013
  • image 02
    Volume 2, No.S1, 2013
  • image 02
    Volume 2, No.3, 2013
  • image 02
    Volume 2, No.4, 2013
  • image 02
    Volume 3, No.1, 2014
  • image 02
    Volume 3, No.2, 2014
  • Volume 3, No3
    Volume 3, No.3, 2014
  • Volume 3, No4
    Volume 3, No.4, 2014
  • Volume 4, No1
    Volume 4, No.1, 2015
  • Volume 4, No2
    Volume 4, No.2, 2015
  • Volume 4, No3
    Volume 4, No.3, 2015
  • Volume 4, No4
    Volume 4, No.4, 2015
  • Volume 5, No1
    Volume 5, No.1, 2016
  • image 01
  • image 02
  • Login|Register
  • Chem Sci Trans., 2016, 5(4),  pp 1049-1055  

    DOI:10.7598/cst2016.1308

    Research Article

    Method Development and Validation RP-HPLC Method for the Simultaneous Estimation of Levofloxacin Hemihydrate and Cefpodoxime Proxetil in Pharmaceutical Dosage Form

  • ANIL KUMAR VEERAGONI*, RANJEETH REDDY K, MALLADI SRINIVAS REDDY, CHILAGANI ARUN KUMAR, VASUDEVA MURTHY SINDGI and SHOBA RANI SATLA
  • Department of Pharmaceutical Analysis, Pathfinder Institute of Pharmacy Education and Research, Warangal-Telangana, India
    Department of Pharmaceutical Analysis and Quality Assurance, Vaageswari College of Pharmacy, Karimnagar-505481, Telangana State, India
    Jayamukhi College of Pharmacy, Narsampet Warangal, Telangana, India
    Centre for Pharmaceutical Sciences, Institute of Science and Technology, JNTUH, Hyderabad, Telangana, India
  • Abstract

    A new simple, sensitive, accurate and precise method has been developed for the simultaneous estimation of Levofloxacin hemihydrate and Cefpodoxime proxetil in pharmaceutical dosage form by RP-HPLC. After optimization good chromatographic separation was achieved by isocratic mode with a mixture of K2HPO4 and methanol pH 8 in the ratio of 40:60 v/v as the mobile phase with YMC C18 (250 mm x 4.6 mm i.d x 5 μm) column as stationary phase at flow rate of 1mL/min and detection wavelength of 275 nm. The retention time of Levofloxacin hemihydrate and Cefpodoxime proxetil were found to be 2.8 and 3.9 min respectively. The linearity of this method was found in the concentration range of 250 μg/mL to 750 μg/mL for Levofloxacin hemihydrate and 200 μg/mL to 600 μg/mL for Cefpodoxime proxetil. The method was extensively validated according to ICH guidelines Q2B for Linearity, Range, Accuracy, Precision, Specificity and Robustness

    Keywords

    High performance liquid chromatography, Gudcef-L, Levofloxacin hemihydrates, Cefpodoxime proxetil

    This article has been viwed 322 times

      

    Citations for this article 0

      

    No Citations