Abstract

Simple, precise and accurate HPLC method for diethylcarbamazine citrate and chlorpheniramine maleate was developed. The combination of DEC and CPM used effectively used to treat filariasis, tropical eosinophilia without causing allergic manifestations and is existing in tablet dosage form. HPLC separation was performed with a hypersil ODS-C18 (5μ, 250 mm x 4.6 mm, i.d) as a stationary phase and methanol: buffer (55:45v/v) as mobile phase, at a flow rate of 1.0 mL/min, UV detection was performed at 254 nm. The retention time of chlorpheniramine maleate and diethylcarbamazine citrate were found to be 2.091 and 4.74 min respectively. Results of analysis were validated by recovery studies. Outcome of studies showed that the projected RP-HPLC method is simple, rapid, accurate and precise which can be used for regular determination of diethylcarbamazine citrate and chlorpheniramine maleate in bulk and its pharmaceutical dosage form.

Keywords

Diethylcarbamazine citrate, Chlorpheniramine maleate, HPLC