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  • Chem Sci Trans., 2017, 6(4),  pp 577-595  

    DOI:10.7598/cst2017.1381

    Research Article

    Visible Spectrophotometric Determination of Metochlopramide Hydrochloride at Trace Levels in Various Samples after Cloud Point Extraction Using Derivatization Reaction

  • ZUHAIR A-A KHAMMAS* and HAWRAA M. ABDULKATEEM
  • Department of Chemistry, College of Science for Women, University of Baghdad, Jadiyriah, Baghdad, Iraq
  • Abstract

    Two new methods have been established based on extraction and determination of drug metochlopramide hydrochloride (MCP.HCl) in different samples, by using surfactant as extracting medium and visible spectrophotometer as a detection system. The methods are depended on the reaction of diazonium MCP.HCl with (A) 8-hydroxyquinoline (8-HQ) and (B) phenylephrine hydrochloride (PHE) in alkaline media to form azo dyes products which can be extracted into micelles of triton x-114 and drug MCP.HCl determined in the visible region at λmax of 530 and 490 nm respectively. All parameters that influence on diazotization coupling reaction between the drug and reagents as well as for extraction efficiency of the colored products are examined by using the conventional optimization. The results have shown that the enrichment factors of 217.7 and 26.98 fold, achieving the detection limit of 0.019 and 0.24 μgmL-1, with linear range of 0.1-0.7 μgmL-1 (r = 0.9996) and 0.5-11.0 μgmL-1 (r=0.9998) for the drug MCP.HCl in method A and B respectively. The mean percent recovery was 98.20±2.71% in drug vial sample and 99.37±2.41 % in tap water and the precision (RSD%) ranged between 3.53-1.21% and 0.97-0.11% for MCP.HCl in method A and B, respectively. The developed methods were compared with reported methods published in chemical literature and used for the determination of MCP.HCl in different samples

    Keywords

    Metochlopramide hydrochloride, 8-Hydroxyquinoline, Phenylephrine hydrochloride, Diazotization-coupling reaction, Cloud point extraction, Visible- spectrophotometry

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