• image 01
    Volume 1, No.1, 2012
  • image 02
    Volume 1, No.2, 2012
  • image 01
    Volume 1, No.3, 2012
  • image 02
    Volume 2, No.1, 2013
  • image 02
    Volume 2, No.2, 2013
  • image 02
    Volume 2, No.S1, 2013
  • image 02
    Volume 2, No.3, 2013
  • image 02
    Volume 2, No.4, 2013
  • image 02
    Volume 3, No.1, 2014
  • image 02
    Volume 3, No.2, 2014
  • Volume 3, No3
    Volume 3, No.3, 2014
  • Volume 3, No4
    Volume 3, No.4, 2014
  • Volume 4, No1
    Volume 4, No.1, 2015
  • Volume 4, No2
    Volume 4, No.2, 2015
  • Volume 4, No3
    Volume 4, No.3, 2015
  • Volume 4, No4
    Volume 4, No.4, 2015
  • Volume 5, No1
    Volume 5, No.1, 2016
  • image 01
  • image 02
  • Login|Register
  • Chem Sci Trans., 2020, 9(1),  pp 23-29  

    DOI:10.7598/cst2020.1709

    Research Article

    Development of Analytical Method Protocol for the Separation and Quantitation of Aflatoxins in Cashew

  • S.P.SAPTALE1*, S.K.PATEL2, SHUJAT QUADRI3 and A.G.DAREKAR4
  • 1Sinhgad Academy of Engineering, Kondhwa (Bk.), Pune, India,
    2Darsh Mahavidyalaya, Omerga, India
    3Maulana Azad College of Arts, Commerce & Science, Aurangabad, India
    4Dhole Patil College of Engineering, Kharadi, Pune, India
  • Abstract

    Aflatoxins are mycotoxins, structurally related compounds produced as secondary metabolites by Aspergillus molds, primarily flavus and parasiticus. Aflatoxins occur naturally in peanuts, cottonseed, corn and dried chili pepper, as well as many mixed or processed foods and feeds. A simple, sensitive and robust HPLC method with post-column derivatization and Ultraviolet detection was used to analyze Aflatoxins B1, B2, G1, and G2 in ground peanuts. Although more than a dozen aflatoxins exist, the four major toxins of interest are B1, B2, G1 and GZ2. They are designated according to their absorption properties in the UV region of the spectrum. Aflatoxin B1 and B2 emit radiation corresponding to blue wavelength, while G1 and G2 emit yellow-green wavelength. The extracts were cleaned using Solid Phase Extraction method (SPE) preferably over immuno affinity columns (IAC) as the former give better recovery of aflatoxins than the latter one even with most challenging matrices and other methods like Soxhlet extraction and Accelerated Solvent Extraction. Further no interferences were found to be present after the cleanup of the sample matrix.

    Keywords

    Mycotoxins, Aflatoxins, Molds, RP-HPLC, Vomitoxin, A. Flavus, Fusarium

    This article has been viwed 153 times

      

    Citations for this article 0

      

    No Citations