Momardica Charantia L. Var. Jhalri (MCJ) is the member of lectins from family Cucurbitaceae. Haemagglutination of purified MCJ lectin was carried out by titration assay with human and animals viz. rabbit, sheep, goat, pig, buffalo and dog erythrocytes. The lectin showed positive haemagglutination test for trypsin treated and non-treated erythrocytes of human blood group ‘O’ and rabbit both. Trypsin treated erythrocytes of human blood group ‘O’ showed the enhanced agglutination while that of rabbit, reduced agglutination. The haemagglutination units(HUs) for untreated and trypsin treated erythrocytes in case of human blood group ‘O’ were found to be 0.043 µg/mL and 0.0107 µg/mL while in case of rabbit erythrocytes these were 0.0215 µg/mL and 0.175 µg/mL respectively.
Lectin, Haemagglutination, Erythrocytes, Trypsin, Titration