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  • Chem Sci Trans., 2013, 2(S1),  pp S1-S6  

    DOI:10.7598/cst2013.296

    Research Article

    RP-HPLC Method for Simultaneous Estimation of Atenolol, Hydrochlorothiazide and Losarton in Tablet Dosage form

  • R. SHARMA, S. KHANNA and G. P. MISHRA
  • School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshshila Campus, Khandwa Road, Indore-452017, M.P., India
  • Abstract

    The present work describes a simple, sensitive, accurate and rapid reverse phase high performance liquid chromatographic method for simultaneous estimation and validation of atenolol, hydrochlorothiazide and losartan in tablet dosage form. Chromatography was performed on a 25 cm 4.6 mm i.d, 5 m particle, C18 column with use of mixture of (A) acetonitrile, methanol (65:35) and (B) 10 mM sodium dihydrogen phosphate monohydrate buffer with 0.4% v/v triethylamine and mixture of A:B (60:40 v/v) adjusted to pH 3.0 with o-phosphoric acid (5% v/v) was used as a mobile phase at a flow rate of 1.5 mL/min. The chromatograms were recorded at 230 nm. The linearity range was found 1-25 g/mL, 1-25 g/mL and 1-25 g/mL for atenolol, hydrochlorothiazide and losartan respectively.

    Keywords

    RP-HPLC, Simultaneous Estimation, Validation, Atenolol, Hydrochlorothiazide, Losartan

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