Abstract

The present work describes a simple, sensitive, accurate and rapid reverse phase high performance liquid chromatographic method for simultaneous estimation and validation of atenolol, hydrochlorothiazide and losartan in tablet dosage form. Chromatography was performed on a 25 cm × 4.6 mm i.d, 5 µm particle, C18 column with use of mixture of (A) acetonitrile, methanol (65:35) and (B) 10 mM sodium dihydrogen phosphate monohydrate buffer with 0.4% v/v triethylamine and mixture of A:B (60:40 v/v) adjusted to pH 3.0 with o-phosphoric acid (5% v/v) was used as a mobile phase at a flow rate of 1.5 mL/min. The chromatograms were recorded at 230 nm. The linearity range was found 1-25 µg/mL, 1-25 µg/mL and 1-25 µg/mL for atenolol, hydrochlorothiazide and losartan respectively.

Keywords

RP-HPLC, Simultaneous Estimation, Validation, Atenolol, Hydrochlorothiazide, Losartan