Total phenol content and antioxidant activity of methanol extract of dried seeds of Lagerstroemia speciosa were determined. The authenticated samples of seeds were powdered and extracted using methanol. The total phenolic content of extract was estimated by Folin-Ciocalteau method. Radical scavenging and reducing activity of seed extract was determined by DPPH free radical scavenging assay and Ferric reducing assay. The extract was found to possess an appreciable quantity of phenolic substances (325±0.01 µg gallic acid equivalents/mg extract). A dose dependent scavenging activity in DPPH assay with IC50 value of 9.63±0.20 µg/mL was observed. The extract showed concentration dependent reducing activity as revealed by an increase in the absorbance of reaction mixture. The observed activity of seed extract could be related to the presence of phenolic substances.
Lagerstroemia speciosa, Antioxidant, DPPH, Ferric reducing, Folin-Ciocalteau