A novel, accurate and precise RP-UFLC method for determination of xanthinol nicotinate has been developed and validated. Separation was achieved on an Enable C18G column (250 mm × 4.6 mm i.d., 5 µm) using acetonitrile: 10 mM TBAHS (40:60, v/v) as mobile phase at a flow rate of 0.8 mL/min and PDA detection at 274 nm. Linearity was observed in the concentration range of 1.0-60 µg/mL (r2=0.999). The method was validated for accuracy, precision, stability, specificity, robustness and system suitability. Forced degradation was performed by using HCl, NaOH, H2O2, thermal and UV radiation. The method was used successfully for the determination of xanthinol nicotinate in tablet dosage form.
Xanthinol, RP-UFLC, Validation, Stability, Nicotinic acid