A simple and rapid spectrophotometeric procedure has been established for quantization of Ni(II) at trace levels. The reagent isonicotinohydroxamic acid (INHA) reacts with nickel(II) at pH < 10 and form a 1:2 lemon green complex. The complex has a maximum absorption at 610 nm with the molar absorptivity of 1.44x104 L mol-1cm-1. Beerís law is obeyed over the range of 0.32-4.80 μgmL-1. The Sandellís sensitivity for 0.001 absorbance unit is 4◊10-3 μgcm-2. The relative standard deviation at 3.20 μgmL-1 is 1.1% (n = 6). The tolerance limits for interfering ions are discussed. All variables were studied in order to optimize the reaction conditions. The efficacy of the proposed method is shown by the successful application of determining nickel in water, wastewater and alloy and the accuracy was determined by recovery experiment.
Spectrophotometry Nickel, INHA