E-J Chem., 2010, 7(1), pp 180- 184, |
Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC MethodD. ANANTHA KUMAR, G. SRINIVASA RAO and J.V.L.N. SESHAGIRI RAOPharmaceutical Analysis and Quality Assurance Division, A. U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530 003, India.Received 14 July 2009 / Accepted 28 August 2009Abstract: A simple, accurate and reproducible RP-HPLC method has been developed for the simultaneous determination of lamivudine, zidovudine and abacavir in tablet dosage forms. Chromatography was carried out on a HiQ Sil C 18 V column using a mobile phase consisting of 0.01 M potassium dihydrogen ortho-phosphate (pH 3.0) and methanol (55:45 v/v) at a flow rate of 0.8 mL/min. The detection was made at 272 nm and stavudine was used as the internal standard for this study. The retention times for lamivudine, abacavir and zidovudine were found to be 3.8, 6.3, 8.1 min. respectively. The calibration curves were linear over the range 5-250 µg/mL for both zidovudine and abacavir and 5-140 µg/mL for lamivudine. The proposed method was validated as per ICH and USP guidelines and it was found suitable for the routine quality control analysis of the drugs in tablet dosage forms.  Corresponding author email idjvlns@yahoo.com |
