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  • Chem Sci Trans., 2014, 3(2),  pp 510-517  

    DOI:10.7598/cst2014.714

    Research Article

    Stability-Indicating RP-HPLC Method for the Determination of Valaciclovir Hydrochloride in Bulk and Pharmaceutical Dosage Forms

  • DILLIP KUMAR SAHOO and PRAFULLA KUMAR SAHU
  • Department of Pharmaceutical Analysis, Avanthi Institute of Pharmaceutical Sciences, Cherukupally, Bhogapuram, Vizianagaram, Andhra Pradesh-531162, India
    Department of Pharmaceutical Analysis, Raghu College of Pharmacy, Dakamarri, Bheemunipatnam, Visakhapatnam, Andhra Pradesh-531162, India
  • Abstract

    A stability indicating reverse-phase HPLC method was developed for the determination of valaciclovir hydrochloride present in bulk and pharmaceutical dosage forms. The quantification was carried out using hypersil, ODS C18 (2504.6 mm i.d., packed with 5 μ particle size) in an isocratic mode column with mobile phase comprising acetonitrile: phosphate buffer (pH- 3.6) in the ratio of 50:50 (%v/v). The flow rate was at 0.8 mL/min and detection was carried out at 252 nm. The retention time was 2.850 min for valaciclovir hydrochloride and the method produced linear response in the concentration range of 0.5 to 200 μg/mL (R2~0.9999). The recovery studies were also carried out and percent relative standard deviation from reproducibility was below 2%. The limit of detection and limit of quantification for this method were 0.110 μg/mL and 0.363 μg/mL, respectively. The proposed method was statistically evaluated and can be applied for routine quality control analysis of valaciclovir hydrochloride in Pharmaceutical dosage form.

    Keywords

    Valaciclovir, Stability indicating, RPHPLC

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