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  • Chem Sci Trans., 2014, 3(4),  pp 1516-1524  

    DOI:10.7598/cst2014.749

    Research Article

    Determination of Aflatoxins in Sesame, Rice, Millet and Acha from Nigeria using HPLC

  • MAKUN HUSSAINI ANTHONY*, APEH DANIEL OJOCHENEMI, ADEYEMI HENRY RINDE YEMI, NAGAGO TAHIR, OKEKE JOHNBOSCO OKECHUKWU, MUSTAPHA AMINA SAIDU and OYINLOYE BUKUNMI AYOBAMI
  • Biochemistry Department, Federal University of Technology Minna, Niger State, Nigeria
    Family Health International (FHI360) Country Office, Abuja, Nigeria
  • Abstract

    A hundred and twenty (120) Nigerian food commodities including; rice (15), madidi (rice product) (15), millet (15), fura (millet dough) (15), sesame (30) and fonio (30) were collected, subjected to aflatoxin extraction and clean up procedures and analyzed quantitatively for aflatoxins using High performance liquid chromatography. Five of the rice samples contained aflatoxin B1 within the range of 37.26-113.2 µg/kg, for madidi samples, 7 contained AFB1 within the range of 0.20-112.5 µg/kg, 3 contained AFB2 within the range of 0.95-18.40 µg/kg while 6.88 µg/kg and 1.60 µg/kg of the green fluorescing aflatoxins 1 and 2 respectively was found in one sample each. Analysis of sesame samples showed that eight contained AFB1 within the range of 14.71-140.9 µg/kg, while one sample contained 2.61 µg/kg of aflatoxin G1. The millet, millet dough and fonio samples all tested negative for the presence of aflatoxins indicating some degree of resistance. All contaminated rice and sesame samples were at ranges above the EU and Nigerian legislated limits for AFB1 and aflatoxins in food. This portrays associated health consequence to consumers and negative impact on trade of these commodities both locally and internationally.

    Keywords

    Aflatoxin, Rice, Millet, Fonio, Sesame

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