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  • Chem Sci Trans., 2014, 3(3),  pp 1078-1082  

    DOI:10.7598/cst2014.809

    Research Article

    Development and Validation of HPLC Method for the Quantification of Anticancer Peptide

  • JAGATHEESH KALIYAPERUMAL, PAVANKUMAR PADARTHI KOWSALYA RANGASAMY and ELANGOVAN NAMASIVAYAM*
  • Molecular Pharmacology Research Laboratory, Department of Biotechnology, Periyar University, Salem, Tamilnadu-636011, India
  • Abstract

    As the prevalence of cancer persists, the development of new and more advanced therapies remains a main concern in the health research. In this work the development and validation of HPLC method for the quantification of anticancer peptide were checked for analytical validation parameters were accuracy, precision (repeatability and reproducibility), specificity, sensitivity (limit of detection and limit of quantification) and linearity. The accuracy and precision of the method have been tested and the coefficients of variation have been found to be less than 2% according to ICH validation guideline. The linearity range was found to be 5-35 μg/mL, LOD-5.65 μg/mL and LOQ-17.1322 μg/mL respectively. In conclusion, a new RP-HPLC method has been developed and validated successfully for the quantitative analysis for the peptide in this study.

    Keywords

    Cancer, RP-HPLC, Quantitative analysis, Peptide, Analytical method

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