A simple, selective, linear, precise and accurate UV Assay method was developed and validated for rapid assay of Doxycycline hyclate in bulk and pharmaceutical formulation. For this development paddle apparatus were used. The purified water was used as dissolution medium and solvent. Stirring rate was employed at 75 rpm and the withdrawal time was identified as 30 minutes and the filter size was found to be 0.45 μ m at ambient temperature. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Doxycycline hyclate in the rapid and reliable determination in pharmaceutical formulation.
Doxycycline hyclate, RP-HPLC, UV detection, Recovery