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Development of Analytical Method Protocol for the Separation and Analysis of Aflatoxins in Dry Coconut by RP-HPLC-UV

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1TitleTitle of DocumentDevelopment of Analytical Method Protocol for the Separation and Analysis of Aflatoxins in Dry Coconut by RP-HPLC-UV
2CreatorAuthor's name, affiliation, country SHRIKAANT KULKARNI1, SHRIDHAR SAPTALE2, SHUJAT QUADRI4, MADHUKAR PATIL3, FAOZIA QUADRI4 and MAQDOOM FAROOQUI4
1Department of Chemical Engineering, Vishwakarma Institute of Technology, Pune, India
2Sinhgad Academy of Engineering, Kondhwa (Bk.), Pune, India
3Yashwantrao Chavan College of Arts, Commerce & Science, Sillod, Aurangabad, India
4Maulana Azad College of Arts, Commerce & Science, Aurangabad, India
3SubjectDicipline(s) Chemical Science
3SubjectKeywords Mycotoxins, Aflatoxins, Molds, RP-HPLC, Vomitoxin, A. Flavus, Fusarium
4DescriptionAbstract Aflatoxins are mycotoxins, structurally related compounds produced as secondary metabolites by aspergillus molds, primarily flavus and parasiticus. Aflatoxins occur naturally in dry coconut, peanuts, cottonseed, corn, almond, cashew nut and dried chili pepper, as well as many mixed or processed foods and feeds. A simple, sensitive, and robust HPLC method and ultraviolet detection was used to analyze aflatoxins B1, B2, G1 and G2 in dry coconut. Although more number of aflatoxins exists, the four major toxins of interest are B1, B2, G1 and G2. They are designated according to their absorption properties in the UV region of the spectrum. Aflatoxin B1 and B2 emit radiation corresponding to blue wavelength, while G1 and G2 emit yellow-green wavelength. The extracts were cleaned using solid phase extraction method (SPE) preferably over immuno affinity columns (IAC) as the former give better recovery of aflatoxins than the latter one even with most challenging matrices and other methods like soxhlet extraction and accelerated solvent extraction. Further no interferences were found to be present after the cleanup of the sample matrix.
5PublishersOrganizing agency, location WWW Publications, India
6Contributor Sponsor(s) -
7DateDate (YYYY-MM-DD) -
8TypeStatus & genre Peer-reviewed Article
8TypeType
9FormateFile Formate PDF
10IdentifierUniform Resource Identifier Click Here
10IdentifierDigital Object Identifier DOI:10.7598/cst2015.1078
11SourceJournal/conference title; vol., no. (Year)Chemical Science Transactions, Volume  4 , Number  (3), (2015)
12LanuguageEnglish=en en
13RelationSupp.files
14Coverage -
15CopyrightCopyright and permissions
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