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Ascorbic Acid, Total Phenol Content and Antioxidant Activity of Fresh Juices of Four Ripe and Unripe Citrus Fruits

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1TitleTitle of DocumentAscorbic Acid, Total Phenol Content and Antioxidant Activity of Fresh Juices of Four Ripe and Unripe Citrus Fruits
2CreatorAuthor's name, affiliation, country C. REKHA, G. POORNIMA, M. MANASA, V. ABHIPSA, J. PAVITHRA DEVI, H T. VIJAY KUMAR and T R. PRASHITH KEKUDA*
Department of Biochemistry, SRNMN College of Applied Sciences, Balraj Urs Road, Shivamogga-577201, Karnataka, India
*Department of Microbiology, SRNMN College of Applied Sciences, Balraj Urs Road, Shivamogga-577201, Karnataka, India
3SubjectDicipline(s) Chemical Science
3SubjectKeywords Citrus, Ascorbic acid, Antioxidant activity, Total phenolic, Folin-Ciocalteu method
4DescriptionAbstract The ascorbic acid and total phenolic content to antioxidant activity of fresh juices of four ripe and unripe citrus fruits namely Citrus limon, C. reticulata, C. sinensis and C. aurantium were compared. The fruits were collected from local market and the pulp and seed free juices were collected. The pH and total acidity were determined. Ascorbic acid content and total phenolic content of fresh fruit juices were determined by volumetric and Folin-Ciocalteu reagent method respectively. Antioxidant activity of fruit juices was determined by two in vitro assays namely DPPH free radical scavenging assay and Ferric reducing assay. The pH was lesser in unripe fruit juices. Acidity, ascorbic acid and total phenolic contents were high in unripe fruit juices. Ascorbic acid and total phenolic content was high in C. aurantium and C. sinensis respectively. In DPPH assay, C. limon, C. reticulata and C. sinensis exhibited stronger scavenging potential when compared to C. aurantium. Ferric reducing potential was higher in C. sinensis followed by others. Overall, unripe fruit juices have displayed stronger antioxidant activity when compared to ripe fruit juices. In this study, the antioxidant activity of fruit juices was shown to be directly related to the content of ascorbic acid and total phenolics except in case of C. aurantium. The lower antioxidant activity of ripe fruit juices could be due to the possible reduction in the ascorbic acid and total phenolic content during ripening.
5PublishersOrganizing agency, location WWW Publications, India
6Contributor Sponsor(s) -
7DateDate (YYYY-MM-DD) -
8TypeStatus & genre Peer-reviewed Article
8TypeType
9FormateFile Formate PDF
10IdentifierUniform Resource Identifier Click Here
10IdentifierDigital Object Identifier DOI:10.7598/cst2012.182
11SourceJournal/conference title; vol., no. (Year)Chemical Science Transactions, Volume  1 , Number  (2), (2012)
12LanuguageEnglish=en en
13RelationSupp.files
14Coverage -
15CopyrightCopyright and permissions
Chemical Science Transactions | Chem Sci Trans | CST | Online Chemistry Journal | Open Access
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