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Microwave Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Hydrolysis of Palm Oil Mill Effluent (POME) for Optimum Fermentable Sugar Yield

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1TitleTitle of DocumentMicrowave Assisted Alkaline Pretreatment and Microwave Assisted Enzymatic Hydrolysis of Palm Oil Mill Effluent (POME) for Optimum Fermentable Sugar Yield
2CreatorAuthor's name, affiliation, country SAIFUDDINNOMANBHAY and REFALHUSSAIN
Centre of Renewable Energy, University Tenaga Nasional, Jalan IKRAM-UNITEN-43000, Kajang, Selangor, Malaysia
3SubjectDicipline(s) Chemical Science
3SubjectKeywords POME, Bioethanol, Enzymatic hydrolysis, Microwave assisted reaction, Non-thermal effects, Enzyme denaturation
4DescriptionAbstract In the present study, a comparison of microwave assisted alkali pretreatment was tried using oil palm empty fruit bunch. The microwave assisted alkali pretreatment of POME using NaOH, significantly improved the enzymatic saccharification of POME by removing more lignin and hemicellulose and increasing its accessibility to hydrolytic enzymes. Enzymatic hydrolysis of POME suspension by various amylolytic enzymes was investigated to reveal the potential coupling mechanism of Microwave Irradiation-Enzyme Coupling Catalysis (MIECC). It was shown that enzymatic hydrolysis of POME using typical enzymes may successfully was carried out at microwave condition. The MIECC resulted in increasing initial reaction rate by about 3 times. The results testify on specific activation of enzymes by microwaves and prove the existence of non-thermal effect in microwave assisted reactions. Low power microwave irradiation (100W) does not increase the temperature beyond 40 oC and hence denaturation of the enzyme is avoided. The present study has highlighted the importance of well controlled microwave assisted enzymatic reaction to enhance the overall reaction rate of the process.
5PublishersOrganizing agency, location WWW Publications, India
6Contributor Sponsor(s) -
7DateDate (YYYY-MM-DD) -
8TypeStatus & genre Peer-reviewed Article
8TypeType
9FormateFile Formate PDF
10IdentifierUniform Resource Identifier Click Here
10IdentifierDigital Object Identifier
11SourceJournal/conference title; vol., no. (Year)Chemical Science Transactions, Volume  3 , Number  (1), (2014)
12LanuguageEnglish=en en
13RelationSupp.files
14Coverage -
15CopyrightCopyright and permissions
Chemical Science Transactions | Chem Sci Trans | CST | Online Chemistry Journal | Open Access
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