E-Journal of Chemistry

Research Article

E-J.Chem , Volume 4, Number 4, p 611-619

Synthesis of Molecularly Imprinted Polymer and its Molecular Recognition Properties of N-Acetylneuraminic Acid

Department of Chemical Engineering, National Chung Cheng University,
Chiayi, Taiwan.
Department of Biotechnology, National Formosa University,
Huwei, Yunlin, 632, Taiwan.
Department of Life Science, Mingdao University,
Peetow, ChangHua, 52345, Taiwan.

ABSTRACT

A molecular imprinting technique was applied in this work to detect N-acetylneuraminic acid (Neu5Ac) and its analogue structure. Two molecularly imprinted polymers (MIP) were prepared using Neu5Ac as the template molecule, as well as methacrylic acid (MAA) or 4-vinylpyridine (4-Vpy) and ethyleneglycol dimethacrylate (EGDMA) as the functional monomer and cross-linker, respectively. Free radical polymerization was carried out at 4^oC under UV radiation or thermal (60 ^oC) polymerization. MIP thus obtained were ground into 11~25μm and 25-44μm. The binding results from Neu5Ac solution, mannose (Man) solution and N-acetyl-D-mannosamine (ManNAc) solution performed by Neu5Ac-MIP showed specific binding toward Neu5Ac rather than other analogue compounds on the host-guest system. The values of capacity for Neu5Ac-MIP were measured and experimental results were further used for simulation to obtain the binding isotherms. The principal advantage of this method is that Neu5Ac- MIP can recognize Neu5Ac and its analogue compounds.

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